Genome editing methods in animal models.

Area of Science:

• Biotechnology and Genetic Engineering
• Translational Medicine
• Animal Models of Human Disease

Background:

• Genetically engineered animal models are crucial for understanding human disease pathology.
• Traditional gene-targeting methods are time-consuming and expensive.
• The advent of CRISPR-Cas9 has transformed the creation of these models.

Purpose of the Study:

• To review CRISPR-Cas9 strategies for creating animal models of human diseases.
• To describe in vivo delivery methods of CRISPR-Cas9 for therapeutic applications in disease models.
• To summarize existing CRISPR-Cas9-generated animal models and discuss future research directions.

Main Methods:

• Utilizing CRISPR-Cas9 genome editing technology for precise DNA mutation introduction.
• Employing various in vivo delivery systems for CRISPR-Cas9 components.
• Reviewing literature on CRISPR-Cas9 applications in animal model generation and disease research.

Main Results:

• CRISPR-Cas9 enables faster and more cost-effective production of genetically engineered animal models.
• This technology facilitates the precise creation of mutations mimicking human genetic disorders.
• Various in vivo delivery methods have been successfully applied for therapeutic gene editing in disease models.

Conclusions:

• CRISPR-Cas9 is a powerful tool for accelerating the development of animal models for human diseases.
• In vivo CRISPR-Cas9 delivery holds significant promise for therapeutic interventions in disease models.
• Continued advancements in CRISPR technology will further enhance its utility in biomedical research.