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Effect of aortic arterial catheterization on tissue glycogen content
R L Moore1, M R Hilty, T I Musch
1Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
Journal of Applied Physiology (Bethesda, Md. : 1985)
|December 1, 1988
Summary
Aortic catheterization in rats significantly reduces liver glycogen levels. Monitoring food intake-to-body weight ratio aids in determining optimal recovery time, crucial for accurate experimental results.
Area of Science:
- Physiology
- Animal Research
- Metabolic Studies
Background:
- Aortic catheterization is common for hemodynamic and metabolite measurements in rats.
- Previous studies noted effects on body weight, but not quantitatively on liver/muscle glycogen.
- Limited understanding of recovery dynamics post-catheterization.
Purpose of the Study:
- To quantify the impact of aortic catheterization on rat liver and skeletal muscle glycogen.
- To evaluate recovery periods for glycogen concentration after catheterization.
- To identify reliable indicators of post-procedural recovery.
Main Methods:
- Polyethylene (PE-50) catheter insertion into the aorta via the carotid artery in rats.
- Measurement of liver and skeletal muscle glycogen concentrations at various time points.
- Comparison of fixed vs. variable recovery periods based on food consumption-to-body weight ratio (FdWt/BdWt).
Main Results:
- Liver glycogen decreased by 56% 24 hours post-catheterization.
- Variable recovery periods (2-8 days) based on FdWt/BdWt were more effective than a fixed 6-day period.
- FdWt/BdWt ratio predicted liver and diaphragm muscle glycogen recovery.
- Other skeletal muscles showed no significant change in glycogen concentration.
Conclusions:
- Aortic catheterization significantly depletes liver glycogen in rats.
- FdWt/BdWt ratio is a reliable indicator for assessing recovery post-aortic catheterization.
- Individualized recovery assessment is superior to fixed recovery periods for experimental consistency.