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Updated: Dec 26, 2025

Using Next Generation Sequencing to Identify Mutations Associated with Repair of a CAS9-induced Double Strand Break Near the CD4 Promoter
Published on: March 31, 2022
Transcription-dependent DNA double-strand breaks and human disease
Agnese Cristini1, Natalia Gromak1, Olivier Sordet2
1Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
None:
Accumulation of DNA damage in resting cells is an emerging cause of human disease. We identified a mechanism of DNA double-strand break (DSB) formation in non-replicating cells, which strictly depends on transcription. These transcriptional DSBs arise from the twinned processing of R-loops and topoisomerase I and may underlie neurological disorders and cancers.
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