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Related Concept Videos

Autophagy01:27

Autophagy

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Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
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Delivery Pathways to the Lysosome01:36

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Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
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Autophagic Cell Death01:18

Autophagic Cell Death

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Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
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Centrosome Duplication02:25

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The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
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Separation of Sister Chromatids02:17

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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Anaphase Promoting Complex00:50

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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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Related Experiment Video

Updated: Dec 26, 2025

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body
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Doryphagy: when selective autophagy safeguards centrosome integrity.

Valentina Cianfanelli1, Francesco Cecconi1,2,3

  • 1Unit of Cell Stress and Survival, Center for Autophagy, Recycling and Disease (CARD), Danish Cancer Society Research Center, Copenhagen, Denmark.

Molecular & Cellular Oncology
|March 12, 2020
PubMed
Summary
This summary is machine-generated.

A newly identified process called doryphagy selectively removes damaged Centriolar Satellites (CS). This selective autophagy maintains centrosome integrity, preventing genomic instability and inaccurate cell division common in cancer.

Keywords:
AutophagyGABARAPGABARAPL2PCM1centriolar satellitescentrosomedoryphagymitosisselective autophagy

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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Centrosome abnormalities are common in cancer, but the underlying mechanisms remain unclear.
  • Understanding how centrosomes are regulated is crucial for cancer biology.

Purpose of the Study:

  • To identify novel mechanisms that maintain centrosome stability.
  • To investigate the role of autophagy in preventing centrosome-related genomic instability.

Main Methods:

  • Identification and characterization of a novel selective autophagy pathway.
  • Analysis of the role of this pathway in maintaining centrosome structure and function.
  • Investigation of the impact of this pathway on mitosis and genomic stability.

Main Results:

  • Discovery of a new selective autophagy process termed 'doryphagy'.
  • Doryphagy specifically targets Centriolar Satellites (CS) for degradation.
  • This process is essential for preserving centrosome organization and preventing inaccurate mitosis.

Conclusions:

  • Doryphagy is a critical mechanism for maintaining genomic stability by regulating centrosome integrity.
  • Targeting doryphagy could offer new therapeutic strategies for cancers with centrosome abnormalities.