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Updated: Dec 26, 2025
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A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
Published on: April 20, 2018
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TeaMiD: a comprehensive database of simple sequence repeat markers of tea
Himanshu Dubey1, Hukam C Rawal1, Megha Rohilla1
1Indian Council Agricultural Research-National Institute for Plant Biotechnology, Lal Bahadur Sashtri Centre, Indian Agricultural Research Institute, Pusa, New Delhi 110012, India.
Database : the Journal of Biological Databases and Curation
|March 12, 2020
Summary
Developing new tea varieties is challenging due to long breeding cycles. This study identified numerous simple sequence repeat (SSR) markers, aiding marker-assisted breeding for tea improvement.
Area of Science:
- Plant genetics
- Genomics
- Biotechnology
Background:
- Tea (Camellia sinensis) breeding is hindered by high heterozygosity and long generation times.
- Conventional breeding methods are inefficient for tea improvement.
- Limited simple sequence repeat (SSR) marker information exists for tea due to its large genome size.
Purpose of the Study:
- To identify and catalog a comprehensive set of SSR markers for tea.
- To develop a user-friendly database for accessing tea SSR information.
- To facilitate marker-assisted breeding strategies in tea.
Main Methods:
- Utilized recently published tea genomes and various public sequence data (RNA-seq, GSS, ESTs, organelle genomes) to identify SSRs.
- Cataloged validated SSR markers from published literature.
- Selected and validated SSR markers across diverse tea genotypes for polymorphism and diversity analysis.
Main Results:
- Identified over 935,000 SSRs from multiple genomic and transcriptomic resources.
- Validated 82 SSRs, with 27 found to be polymorphic.
- Six primers showed high polymorphism (average five alleles) and were used for diversity analysis in 36 genotypes, yielding high polymorphic information content (0.61-0.76).
Conclusions:
- Developed TeaMiD, a comprehensive database of tea SSR markers from diverse sources.
- The identified SSR markers and the database are valuable resources for accelerating tea genetic research and breeding.
- Marker-assisted breeding strategies can significantly improve tea cultivar development.
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