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Immune Cell Status and Cytokines Profiles in Patients with Acute Retinal Necrosis
Peijun Zhang1,2,3, Shixue Liu1,2,3, Zhujian Wang1,2,3
1Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.
Ocular Immunology and Inflammation
|March 12, 2020
Summary
Acute retinal necrosis (ARN) involves elevated T-helper 17 (Th17) cells and inflammatory cytokines. These findings suggest Th17 cells play a role in ARN immunopathogenesis.
Area of Science:
- Ophthalmology
- Immunology
- Virology
Background:
- Acute retinal necrosis (ARN) is a severe intraocular inflammatory condition.
- Understanding the immune mechanisms underlying ARN is crucial for effective treatment.
Purpose of the Study:
- To evaluate the immune status of patients diagnosed with ARN.
- To identify specific immune cell types implicated in the immunopathogenesis of ARN.
Main Methods:
- Collected peripheral blood and intraocular fluid from 17 ARN patients and 9 controls.
- Utilized flow cytometry for immune cell analysis, rate nephelometry for complement and antibody levels, and cytokine chips for cytokine quantification.
- Statistical analysis was performed using SPSS 23.0, with p < 0.05 considered significant.
Main Results:
- ARN patients exhibited a higher proportion of T-helper 17 (Th17) cells in serum (p=0.034).
- Elevated levels of Th17-associated cytokines (IL-6, IL-17, IL-17 F, IL-21, IL-22) were observed in both serum and aqueous humor (AH) of ARN patients.
Conclusions:
- Th17 cells are implicated in the immunopathogenesis of ARN.
- Increased inflammatory cytokines and immune cells in serum and AH suggest a significant role in ARN development.
