GABA as a potential transmitter in lizard photoreceptors: immunocytochemical and biochemical evidence

The retina of the desert night lizard, Xantusia vigilis, was examined for immunoreactivity to antibodies against gamma-aminobutyric acid and L-glutamate decarboxylase. At the electron microscopic level it was found that a distinct population of the photoreceptor cells was immunoreactive to both antibodies. Computer-assisted reconstruction of serial sections positively identified the immunoreactive receptors as cones. These cones constituted 15% of the photoreceptors in the retinal sections, and they were morphologically distinct. The mean diameter of the labeled cone synaptic pedicles was 5.8 micron whereas that of the unlabeled pedicles was 7.9 micron, a statistically significant difference. L-glutamate decarboxylase was extracted from the lizard brain, positively identified radiometrically, and shown by immunodiffusion to crossreact with the antibody used for localization. The authors suggest that the immunoreactive cones synthesize and accumulate gamma-aminobutyric acid. Whether or not it is used by those cones as a neurotransmitter should be tested directly.