Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.3K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.3K
Replication in Eukaryotes01:29

Replication in Eukaryotes

16.8K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
16.8K
Replication in Eukaryotes02:31

Replication in Eukaryotes

201.9K
Overview
201.9K
Chromosome Replication02:31

Chromosome Replication

10.3K
Before a cell can divide, it must accurately replicate all of its chromosomes, including the DNA and its associated histone and non-histone proteins.  This process begins at numerous origins of replication during the S phase of the cell cycle in each of a cell’s chromosomes simultaneously. Certain nucleotides can act as origins of replication, but these sequences are not well defined - especially in complex, multi-cellular, eukaryotic species. The length of DNA that spans an origin...
10.3K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

9.9K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.9K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Cytosine‑Bulged (3+1) Hybrid G‑Quadruplex Formed by the Chicken DNA Replication Origin.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Upregulation of ATP-purinergic P2x2 receptors in the cochlea over-amplifies hearing sensitivity leading to hyperacusis and attenuation by antagonists.

bioRxiv : the preprint server for biology·2026
Same author

The predictive accuracy of axial length to corneal curvature radius ratio for myopia in children and adolescents.

Frontiers in medicine·2026
Same author

Epicardial muscle bundles in persistent atrial fibrillation: from overlooked bystanders to arrhythmogenic modulators.

Journal of interventional cardiac electrophysiology : an international journal of arrhythmias and pacing·2026
Same author

A Fully Potentiometric Electronic Tongue Enabling Comprehensive Physical and Chemical Sensations.

ACS applied materials & interfaces·2026
Same author

Associations between the C-reactive protein-triglyceride glucose index and the incidence and progression trajectory of cardiometabolic multimorbidity: a multi-state model study.

Cardiovascular diabetology·2026

Related Experiment Video

Updated: Dec 26, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

28.2K

An Essential and Cell-Cycle-Dependent ORC Dimerization Cycle Regulates Eukaryotic Chromosomal DNA Replication.

Aftab Amin1, Rentian Wu2, Man Hei Cheung2

  • 1School of Chinese Medicine and Department of Biology, Hong Kong Baptist University, Hong Kong, China; Division of Life Science, Center for Cancer Research, and State Key Lab of Molecular Neuroscience, Hong Kong University of Science and Technology, Hong Kong, China.

Cell Reports
|March 12, 2020
PubMed
Summary

The origin recognition complex (ORC) uses a dimerization cycle to ensure DNA replication occurs only once per cell cycle. This cycle facilitates pre-replication complex loading and protects newly replicated origins from re-licensing.

Keywords:
DNA replicationORC dimerizationcell-cycle controlpre-RC formationreplication licensing

More Related Videos

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

1.8K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.8K

Related Experiment Videos

Last Updated: Dec 26, 2025

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

28.2K
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
08:40

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Published on: October 21, 2022

1.8K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

14.8K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Eukaryotic DNA replication licensing ensures genome duplication occurs precisely once per cell cycle.
  • The Origin Recognition Complex (ORC) binds replication origins and loads proteins to form pre-replicative complexes (pre-RCs).
  • The mechanism of ORC loading symmetric MCM double hexamers and its role in protecting newly replicated origins remain unclear.

Purpose of the Study:

  • To elucidate the essential, cell-cycle-dependent ORC "dimerization cycle".
  • To understand how ORC facilitates the loading of symmetric pre-RCs.
  • To investigate ORC's role in marking and protecting nascent replication origins and preventing re-licensing.

Main Methods:

  • The study likely involved biochemical assays and cell-based experiments to investigate ORC structure and function.
  • Analysis of ORC protein interactions and localization throughout the cell cycle was crucial.
  • Investigating the impact of ORC dimerization on MCM loading and origin protection.

Main Results:

  • An essential, cell-cycle-dependent ORC dimerization cycle was identified.
  • This cycle provides a symmetric platform for loading symmetric pre-RCs.
  • The ORC cycle marks and protects nascent sister origins, preventing re-licensing within the same cell cycle.

Conclusions:

  • The ORC dimerization cycle is fundamental for regulating DNA replication.
  • It ensures proper pre-RC formation and prevents re-replication.
  • This mechanism is critical for maintaining genome stability and cell cycle integrity.