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Inner Mongolian Cashmere Goat Secondary Follicle Development Regulation Research Based on mRNA-miRNA Co-analysis.

Wenjing Han1,2, Feng Yang1, Zhihong Wu1

  • 1College of Animal Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia Autonomous Region, 010018, Hohhot, China.

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|March 13, 2020
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This study reveals that microRNA-199a-5p (chi-miR-199a-5p) inhibits the expression of TGF-β2, a key factor in cashmere goat hair follicle development during the fetal period.

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Area of Science:

  • Animal Science
  • Molecular Biology
  • Genetics

Background:

  • Inner Mongolia cashmere goats are vital to the animal fiber industry.
  • Research on the molecular regulation of hair follicle growth exists, but fetal cashmere development is understudied.

Purpose of the Study:

  • To investigate the molecular mechanisms regulating cashmere hair development in fetal Inner Mongolia cashmere goats.
  • To identify microRNAs (miRNAs) and their target genes involved in secondary hair follicle development.

Main Methods:

  • Performed mRNA and miRNA sequencing on 21 skin samples from seven fetal stages (45-135 days).
  • Utilized bioinformatics analysis to identify miRNA-target gene interactions.
  • Employed Dual-Luciferase Reporter Gene System, RT-qPCR, and Western blot to validate interactions, specifically between chi-miR-199a-5p and TGF-β2.

Main Results:

  • Sequencing results for seven genes and five miRNAs showed consistent expression trends with fetal development.
  • A strong negative correlation (-0.84) was observed between TGF-β2 and chi-miR-199a-5p expression.
  • chi-miR-199a-5p was confirmed to inhibit TGF-β2 expression at both mRNA and protein levels in goat fibroblasts.

Conclusions:

  • chi-miR-199a-5p acts as a negative regulator of TGF-β2 during cashmere goat fetal skin development.
  • This finding provides a theoretical basis for understanding miRNA-mediated regulation of hair follicle development in cashmere goats.