Circular RNA circ_0001105 Inhibits Progression and Metastasis of Osteosarcoma by Sponging miR-766 and Activating

Related Experiment Video

Updated: Dec 26, 2025

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.8K

Jie Yang1, Qicai Han2, Chao Li3

  • 1Department of Orthopedics, Zhengzhou Central Affiliated Hospital to Zhengzhou University, Zhengzhou 450052, People's Republic of China.

Oncotargets and Therapy
|March 13, 2020
PubMed
Abstract

Insights

Circular RNAs (circRNAs) like circ_0001105 inhibit osteosarcoma (OS) progression. This study shows circ_0001105, via the miR-766/YTHDF2 pathway, suppresses OS cell viability and tumor growth.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are critical regulators of tumor progression.
  • Osteosarcoma (OS) is a primary bone malignancy with complex regulatory mechanisms.
  • The specific role of circ_0001105 in OS progression requires elucidation.

Purpose of the Study:

  • To investigate the biological functions of circ_0001105 in osteosarcoma (OS).
  • To determine the molecular mechanism underlying circ_0001105's role in OS progression.
  • To assess the potential of circ_0001105 as a therapeutic target in OS.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and in situ hybridization (ISH) for expression analysis.
  • Bioinformatics, dual-luciferase reporter assays, and Western blot to elucidate RNA interactions.
  • In vitro cell assays (CCK-8, colony formation, EdU, transwell, wound healing) and in vivo xenograft models to evaluate functional roles.

Main Results:

  • circ_0001105 and YTHDF2 expression were significantly lower in OS tissues, correlating with poor patient survival.
  • miR-766 was identified as a direct target of circ_0001105 and YTHDF2.
  • Overexpression of circ_0001105 or YTHDF2 suppressed OS cell viability and invasion by regulating miR-766.
  • circ_0001105 overexpression attenuated tumor growth in vivo.

Conclusions:

  • circ_0001105 functions as a tumor suppressor in osteosarcoma.
  • The study elucidates a novel circ_0001105/miR-766/YTHDF2 signaling pathway critical for OS progression.
  • circ_0001105 holds potential as a diagnostic biomarker and therapeutic target for osteosarcoma.
Keywords:
OSYTHDF2circ_0001105miR-766progression

More Related Videos

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

11.0K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.7K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.2K
Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
6.0K