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Related Concept Videos

Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

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As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
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Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
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Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
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The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
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The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
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Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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Related Experiment Video

Updated: Dec 26, 2025

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
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Hec1/Ndc80 Tail Domain Function at the Kinetochore-Microtubule Interface.

Robert T Wimbish1, Jennifer G DeLuca1

  • 1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, United States.

Frontiers in Cell and Developmental Biology
|March 13, 2020
PubMed
Summary

The NDC80 complex and its Hec1 tail domain are crucial for stable kinetochore-microtubule attachments during cell division. Recent research offers a unified view of the Hec1 tail domain

Keywords:
Hec1NDC80kinetochoremicrotubulemitosis

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biophysics

Background:

  • Mitotic cell division relies on precise chromosome-microtubule attachments.
  • Kinetochores, protein structures on chromosomes, mediate these crucial attachments.
  • Attachment stability is vital for accurate mitosis, requiring tight regulation.

Purpose of the Study:

  • To review the function of the NDC80 complex at the kinetochore-microtubule interface.
  • To highlight the specific role of the Hec1 tail domain in attachment regulation.
  • To synthesize recent findings into a unified understanding of Hec1 tail domain function.

Main Methods:

  • Literature review of studies on kinetochore-microtubule interactions.
  • Analysis of research on the NDC80 complex and its subunits.
  • Focus on the N-terminal 'tail' domain of the Hec1 subunit.

Main Results:

  • The NDC80 complex is a core component of kinetochore-microtubule attachments.
  • The Hec1 tail domain plays a significant role in generating and regulating these attachments.
  • Recent studies provide a cohesive perspective on the Hec1 tail domain's contributions.

Conclusions:

  • The Hec1 tail domain is a key regulator of kinetochore-microtubule attachment dynamics.
  • Understanding this domain is essential for comprehending error-free mitotic progression.
  • This review consolidates current knowledge on the Hec1 tail domain's multifaceted roles.