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Related Experiment Videos

Analysis of drug penetration through skin considering donor concentration decrease.

M Hashida1, H Okamoto, H Sezaki

  • 1Faculty of Pharmaceutical Sciences, Kyoto University, Japan.

Journal of Pharmacobio-Dynamics
|September 1, 1988
PubMed
Summary
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A new diffusion model accurately predicts in vitro drug penetration through skin by accounting for decreasing donor concentration. This model yields parameters for drug diffusion and partitioning, enabling calculation of mean transit time (MTT) as an index of skin penetrability.

Area of Science:

  • Pharmacokinetics and Drug Delivery
  • Biophysical Modeling
  • Dermal Absorption Studies

Background:

  • Understanding in vitro drug penetration through skin is crucial for pharmaceutical development.
  • Existing models often do not fully account for dynamic changes in drug concentration within the donor compartment.
  • Accurate modeling enhances prediction of drug disposition in dermal tissues.

Purpose of the Study:

  • To develop and validate a diffusion model for in vitro skin penetration that incorporates drug concentration decrease in the donor solution.
  • To identify key parameters governing drug diffusion and partitioning within the skin.
  • To introduce and evaluate the mean transit time (MTT) as a metric for drug penetrability.

Main Methods:

  • Construction of a diffusion model incorporating drug concentration depletion in the donor solution.

Related Experiment Videos

  • Derivation of Laplace transforms for drug amounts in donor, skin, and receptor compartments over time.
  • Non-linear least squares fitting of in vitro penetration data using an inverse Laplace transform algorithm (MULTI(FILT)).

    • Main Results:

    • The model successfully estimated drug amounts remaining in the donor solution and skin.
    • Two parameters, representing drug diffusion and partitioning, were accurately determined.
    • The mean transit time (MTT) was calculated using the derived parameters.

    Conclusions:

    • The developed diffusion model provides a robust framework for analyzing in vitro drug skin penetration.
    • The derived parameters offer quantitative insights into drug diffusion and partitioning kinetics.
    • Mean transit time (MTT) serves as a valuable and reliable index for assessing drug penetrability through the skin.