Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.3K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.3K
Tissue Homogenization and Cell Lysis01:32

Tissue Homogenization and Cell Lysis

9.7K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
9.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FedAttn-Credit: attention-augmented federated learning with adaptive differential privacy for rural inclusive finance credit assessment.

Scientific reports·2026
Same author

Author Correction: Multi-omics profiling reveals tumor microenvironment characteristics linked to immunotherapy response and prognosis in non-small cell lung cancer.

NPJ precision oncology·2026
Same author

Flexible Surface Acoustic Wave (SAW) Magnetic Sensor Based on Terfenol-D Grating-Arrayed Thin Polymer Film.

Micromachines·2026
Same author

Investigation of monotherapeutic high-intensity focused ultrasound therapy for thrombolysis in highly occluded vessels: an<i>in vitro</i>study.

Physics in medicine and biology·2026
Same author

Multi-omics profiling reveals tumor microenvironment characteristics linked to immunotherapy response and prognosis in non-small cell lung cancer.

NPJ precision oncology·2026
Same author

Influence of Preoperative Cognitive Function, Self-Efficacy, and Postoperative Psychological Counselling on Anxiety and Depression Levels in Patients Undergoing Botulinum Toxin Injections.

Actas espanolas de psiquiatria·2026

Related Experiment Video

Updated: Dec 26, 2025

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System
08:19

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Published on: May 9, 2021

2.6K

Cell Lysis Based on an Oscillating Microbubble Array.

Xiufang Liu1,2, Jinyuan Li1,2, Liangyu Zhang1

  • 1College of Medicine and Biological Information Engineering, Northeastern University, 195 Innovation Road, Shenyang 110016, China.

Micromachines
|March 14, 2020
PubMed
Summary
This summary is machine-generated.

Researchers developed a microbubble array for efficient cell lysis, releasing intracellular DNA, RNA, and proteins. This method offers a fast, cost-effective alternative to commercial kits for diagnostics and research.

Keywords:
cell lysisshear stressstable cavitationultrasound bioeffects

More Related Videos

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)&#8211;Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

12.1K
Introduction to the Ultrasound Targeted Microbubble Destruction Technique
08:24

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

Published on: June 12, 2011

16.0K

Related Experiment Videos

Last Updated: Dec 26, 2025

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System
08:19

Induction of Microstreaming by Nonspherical Bubble Oscillations in an Acoustic Levitation System

Published on: May 9, 2021

2.6K
A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)&#8211;Cell Interaction and the Resultant Bioeffects at the Single-cell Level
11:14

A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level

Published on: January 10, 2017

12.1K
Introduction to the Ultrasound Targeted Microbubble Destruction Technique
08:24

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

Published on: June 12, 2011

16.0K

Area of Science:

  • Biotechnology
  • Cell Biology
  • Biomedical Engineering

Background:

  • Cell lysis is crucial for releasing intracellular substances like DNA, RNA, and proteins.
  • These substances are vital for applications in cancer diagnostics and drug screening.
  • Current methods for cell lysis can be time-consuming or expensive.

Purpose of the Study:

  • To develop a novel microbubble array device for efficient and high-throughput cell lysis.
  • To investigate the parameters influencing cell lysis efficiency using oscillating microbubbles.
  • To compare the performance of the developed device with commercial cell lysis kits.

Main Methods:

  • Fabrication of a microbubble array device for generating shear stress.
  • Induction of cell lysis through oscillating microbubbles at varying input voltages and sonication times.
  • Analysis of extracted DNA using DNA agarose-gel electrophoresis.

Main Results:

  • The microbubble array effectively released intracellular substances from cells via shear stress.
  • Lysis efficiency increased with higher input voltage and longer sonication times.
  • DNA agarose-gel electrophoresis confirmed sufficient DNA extraction, comparable to commercial kits.

Conclusions:

  • The developed microbubble array provides an efficient, rapid, and cost-effective method for cell lysis.
  • This technology holds significant potential for single-cell sequencing, disease diagnosis, and stem cell therapy.
  • The device offers a versatile tool for various cell biology research applications.