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Updated: Dec 26, 2025

Millisecond Hydrogen/Deuterium-Exchange Mass Spectrometry for the Study of Alpha-Synuclein Structural Dynamics Under Physiological Conditions
Published on: June 23, 2022
Salient Features of Monomeric Alpha-Synuclein Revealed by NMR Spectroscopy
Do-Hyoung Kim1, Jongchan Lee2, K H Mok3,4
1Core Facility Management Center, Division of KRIBB Strategic Projects, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Korea.
Abstract:
Elucidating the structural details of proteins is highly valuable and important for the proper understanding of protein function. In the case of intrinsically disordered proteins (IDPs), however, obtaining the structural details is quite challenging, as the traditional structural biology tools have only limited use. Nuclear magnetic resonance (NMR) is a unique experimental tool that provides ensemble conformations of IDPs at atomic resolution, and when studying IDPs, a slightly different experimental strategy needs to be employed than the one used for globular proteins. We address this point by reviewing many NMR investigations carried out on the α-synuclein protein, the aggregation of which is strongly correlated with Parkinson's disease.
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