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Updated: Dec 26, 2025
05:45
Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
Published on: July 31, 2017
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Tumor mutational burden is associated with poor outcomes in diffuse glioma
Lihong Wang1, Jia Ge1, Yang Lan1
1Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University (Army Medical University) and Key Laboratory of Tumor Immunopathology, Ministry of Education of China, Chongqing, 400038, China.
BMC Cancer
|March 14, 2020
Summary
Tumor mutational burden (TMB) is linked to poorer outcomes in diffuse glioma patients, with higher TMB correlating to shorter survival. Targeted sequencing panels did not reflect this prognostic association.
Area of Science:
- Oncology
- Genomics
- Biomarker Discovery
Background:
- Tumor mutational burden (TMB) is a key biomarker for immune checkpoint therapy and prognosis.
- The prognostic impact of TMB and its correlation between exome and targeted sequencing in glioma remain underexplored.
Purpose of the Study:
- To investigate the clinical significance of TMB in diffuse glioma.
- To evaluate the correlation between TMB derived from exome sequencing and targeted sequencing.
Main Methods:
- Analysis of somatic mutations and clinical data from TCGA (training) and CGGA (validation) cohorts.
- Descriptive, correlational, enrichment map, and gene set enrichment analyses (GSEA) were performed.
- TMB was assessed using whole-exome sequencing and targeted sequencing panels.
Main Results:
- Higher TMB was observed in glioblastoma-associated genes compared to lower-grade glioma genes.
- Elevated TMB correlated with shorter overall survival, higher tumor grade, older age, and specific molecular subtypes.
- TMB was an independent prognostic factor; cell cycle pathways were enriched in TMB-high gliomas.
- Correlation between exome- and targeted sequencing-based TMB was moderate; panel-based TMB lacked prognostic value.
Conclusions:
- TMB is associated with adverse outcomes in diffuse glioma, potentially due to increased proliferative activity.
- The prognostic value of TMB in glioma is not reliably captured by standard targeted sequencing panels.
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