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Draft Genome Sequence of Paenibacillus polymyxa DSM 292, a Gram-Positive, Spore-Forming Soil Bacterium with High

Simon Heinze1, Ilias Lagkouvardos2, Wolfgang Liebl1

  • 1Department of Microbiology, Technical University of Munich, Freising, Germany.

Microbiology Resource Announcements
|March 14, 2020
PubMed

Insights

The draft genome sequence of Paenibacillus polymyxa DSM 292, a soil bacterium known for antibiotic production, was determined. Its low proteolytic activity makes it a promising candidate for heterologous protein production.

Area of Science:

  • Microbiology
  • Genomics
  • Biotechnology

Background:

  • Paenibacillus polymyxa DSM 292, isolated in 1947, is known for antibiotic production.
  • The strain exhibits low proteolytic activity, suggesting potential for protein expression.
  • Heterologous protein production requires suitable microbial hosts with minimal protein degradation.

Purpose of the Study:

  • To determine the draft genome sequence of Paenibacillus polymyxa DSM 292.
  • To provide genomic data for evaluating DSM 292 as a host for heterologous protein production.

Main Methods:

  • Illumina MiSeq paired-end sequencing was employed to generate the draft genome sequence.
  • Bioinformatic analysis was performed on the sequencing data.

Main Results:

  • The draft genome sequence of Paenibacillus polymyxa DSM 292 was successfully established.
  • Genomic information was obtained for further characterization of the strain's capabilities.

Conclusions:

  • The availability of the DSM 292 genome sequence facilitates its assessment as a host for heterologous protein production.
  • Further research can leverage this genomic data to explore and optimize protein expression in Paenibacillus polymyxa.

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