Related Experiment Video

Updated: Dec 26, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.7K

Long Noncoding RNA CTC Inhibits Proliferation and Invasion by Targeting miR-146 to Regulate KIT in Papillary Thyroid

Baochun Liao1, Shi Liu2, Jiafeng Liu1

  • 1Department of Thyroid Surgery, First Affiliated Hospital of Gannan Medical University, Ganzhou, 341000, China.

Scientific Reports
|March 14, 2020
PubMed

Insights

Long non-coding RNA CTC inhibits papillary thyroid carcinoma (PTC) progression and chemoresistance by acting as a microRNA-146 decoy, targeting KIT. Downregulation of CTC and KIT, with increased miR-146, was observed in PTC tissues.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in various diseases, but their role in papillary thyroid carcinoma (PTC) remains underexplored.
  • Understanding lncRNA functions is crucial for developing novel therapeutic strategies in oncology.

Purpose of the Study:

  • To investigate the biological relevance and regulatory mechanisms of lncRNAs, specifically CTC, in papillary thyroid carcinoma (PTC).
  • To elucidate the molecular pathway involving CTC, microRNA-146 (miR-146), and KIT in PTC progression and chemoresistance.

Main Methods:

  • In vitro and in vivo assays were employed to assess the role of CTC in PTC cell migration and invasion.
  • Mechanistic studies explored the interaction between CTC, miR-146, and KIT expression.
  • Quantitative analysis of CTC, miR-146, and KIT levels in clinical PTC specimens compared to normal thyroid tissues.

Main Results:

  • CTC was identified as a negative regulator of PTC cell migration and invasion.
  • CTC functions as a miR-146 decoy, de-repressing KIT expression.
  • Downregulation of CTC and KIT, coupled with elevated miR-146, was observed in PTC tissues, correlating with disease progression.

Conclusions:

  • CTC acts as a competing endogenous RNA (ceRNA) to inhibit PTC progression and chemoresistance via the miR-146/KIT axis.
  • CTC represents a potential therapeutic target for suppressing PTC progression.

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.5K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
5.5K