Toxicopathological, Cytogenetic Effects of Acetothioamide on Female Albino Mice Reproductive System
Anas A Humadi1, Bushra I Al-Kaisei2, Taghreed J Humadai2
1Department of Pathology, College of Veterinary Medicine, University of Diyala, Diyala, Iraq.
Aim:
To determine toxicopathological and cytogenetic effects of Acetothioamide (ATA) on the female reproductive system.
Methods:
Twenty albino female mice were divided equally into two groups: the first group (control) fed with diet pellet. The second group (treated) were inoculated intraperitoneally with a single dose of ATA (100 mg/kg Bw) for 15 days. All mice were sacrificed at the end of the experiment and blood was collected for evaluation of (FSH and LH), serum peroxy nitrate radical concentration. Cytogenetic analysis (chromosomal aberration, micronuclei, mitotic index and blast index) and the histopathological examination on ovary and uterus were done.
Results:
ATA causes significant reduction (p < 0.0001) for FSH, LH and serum peroxy nitrate radical concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were reported among treated females mainly in uterus tissue and ovary.
Conclusion:
ATA causes significant reduction for FSH, LH and serum peroxy nitrate concentration among treated females. Oophoritis, pyometria, thrombosis and endometrial hyperplasia with granulomatous reaction were the main pathological changes in uterus tissue and ovary among treated females.
Insights
Acetothioamide (ATA) exposure significantly reduced reproductive hormones (FSH, LH) and caused reproductive organ damage in female mice. Pathological changes included oophoritis, pyometria, and endometrial hyperplasia.
Area of Science:
- Toxicology
- Reproductive Biology
- Cytogenetics
Background:
- Acetothioamide (ATA) is a chemical compound with potential toxicological effects.
- The female reproductive system is sensitive to environmental and chemical exposures.
- Understanding the impact of ATA on reproductive health is crucial.
Purpose of the Study:
- To investigate the toxicopathological and cytogenetic effects of Acetothioamide (ATA) on the female reproductive system.
- To assess the impact of ATA on reproductive hormone levels and oxidative stress markers.
- To evaluate histopathological and cytogenetic alterations in the ovaries and uterus.
Main Methods:
- Female albino mice were divided into control and ATA-treated groups.
- ATA was administered intraperitoneally at a dose of 100 mg/kg Bw for 15 days.
- Reproductive hormones (FSH, LH), peroxy nitrate radical concentration, histopathology, and cytogenetic analyses were performed.
Main Results:
- ATA treatment led to a significant reduction in FSH, LH, and serum peroxy nitrate radical concentration (p < 0.0001).
- Histopathological examination revealed oophoritis, pyometria, thrombosis, and endometrial hyperplasia with granulomatous reactions in the ovaries and uterus.
- Cytogenetic analysis indicated significant alterations in chromosomal aberration, micronuclei, mitotic index, and blast index.
Conclusions:
- Acetothioamide (ATA) exerts significant toxicopathological effects on the female reproductive system.
- ATA exposure reduces key reproductive hormones and induces oxidative stress.
- Histopathological and cytogenetic damage to the ovaries and uterus highlights the reproductive toxicity of ATA.
