Genetic variant of SPARC gene and its association with growth traits in Chinese cattle
Danyang Zhang1, Jiawei Xu1, Peng Yang1
1College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Abstract:
SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T C). A total of 616 samples of four breeds of Qinchuan cattle (QC, ), Xianan cattle (XN, ), Pinan cattle (PN, ) and Jiaxian cattle (JX, ) were analyzed and identified with ARMS-PCR. In addition, we correlated SNP with growth traits and showed significant correlation with growth traits such as rump length, hip width, and body length ( ). Moreover, we tested the SPARC gene expression level in different tissues belonging to XN adult cattle ( ) and found its high expression in muscle tissues (relative to the kidney). Further, we found the SNP is able to increase the SPARC expression level in skeletal muscle ( ). According to statistical data, this SNP site may be applied to a molecular marker of an early marker-assisted selection for early growth of beef cattle.
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