Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Dec 26, 2025

Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.7K

Enhanced chromosome extraction from cells using a pinched flow microfluidic device.

Haidong Feng1, Matthew Hockin2, Shuhua Zhang2

  • 1State of Utah Center of Excellence for Biomedical Microfluidics, University of Utah, Salt Lake City, UT, USA.

Biomedical Microdevices
|March 14, 2020
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026
Same author

Rosavin alleviates COPD via inhibition of IL-17-enriched NET formation and NF-κB signaling.

Biology direct·2026
Same author

How Uncertainty in Illness Affects Death Anxiety in Patients With Early-Stage Breast Cancer: Based on the Common-Sense Model of Self-Regulation.

Cancer nursing·2026
Same author

Inhibiting astrocyte activation is effective in triptan-induced medication-overuse headache model and prolonged hyperalgesia.

Headache·2026
Same author

Mechanically robust PEDOT:PSS hydrogel <i>via</i> mild liquid metal crosslinking.

Soft matter·2026
Same author

Methodology and baseline characteristics of the China Migraine registry (CHIME): a nationwide, multi-center, prospective cohort study of 11,814 patients.

The journal of headache and pain·2026
This summary is machine-generated.

This study introduces a mild chromosome extraction method using microfluidics to improve intact chromosome yield for research. The optimized process significantly increases chromosome release compared to traditional methods.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Microfluidics

Background:

  • Intact chromosome extraction is vital for transchromosomic research.
  • Conventional methods often result in low yields and chromosome degradation.

Purpose of the Study:

  • To develop a "mild" chromosome extraction process.
  • To enhance intact chromosome preparation for downstream applications.

Main Methods:

  • Combined chemical lysis and mechanical disruption using a microfluidic pinched flow device.
  • Optimization of microfluidic geometry and operational parameters through simulations and experiments.

Main Results:

  • The microfluidic device enhances cell lysis and chromosome release via shear stress.
Keywords:
Cell lysisChromosome extractionMicrofluidic devicePinched flow deviceSample preparation

More Related Videos

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.5K
Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

4.0K

Related Experiment Videos

Last Updated: Dec 26, 2025

Cell Capture Using a Microfluidic Device
29:02

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

5.7K
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.5K
Capturing Chromosome Conformation Across Length Scales
10:15

Capturing Chromosome Conformation Across Length Scales

Published on: January 20, 2023

4.0K
  • Optimized devices achieved a 4-fold increase in chromosome release (8% to 25%).
  • Reduced sample treatment time was observed.

    • Conclusions:

    • The combined chemical-mechanical lysis in microfluidics is effective for intact chromosome extraction.
    • Device design (constriction, channel width) and flow rate are critical for optimizing chromosome release.
    • This method provides higher yields of intact chromosomes for research applications.