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Published on: March 30, 2019
MicroRNA-760 inhibits cell viability and migration through down-regulating BST2 in gastric cancer
Weiyu Liu1, Yan Li1, Shuting Feng1
1Department of Gastroenterology, The People's Hospital of Liaoning Province, Shenyang 110016, People's Republic of China.
Abstract:
Gastric cancer is one of the most common types of carcinoma with a threat to global health. MicroRNA-760 (miR-760) was significantly down-regulated in the primary tumour of patients with advanced gastric cancer. However, the role of miR-760 in gastric cancer is still unclear. Herein, miR-760 was down-regulated in gastric cancer tissues. Moreover, miR-760 overexpression and knockdown were conducted in gastric cancer cells (MGC-803 and SGC-7901) in vitro. The in vitro functional assays proved that miR-760 overexpression reduced cell viability, cell cycle, migration and invasion, promoted apoptosis and suppressed MMP activity in MGC-803 cells. Conversely, miR-760 knockdown led to the opposite in SGC-7901 cells. Notably, bone marrow stromal antigen 2 (BST2) was verified as a target gene of miR-760. MiR-760 mimics down-regulated BST2 level in gastric cancer tissues and in MGC-803 cells, whereas miR-760 inhibitor up-regulated its level in SGC-7901 cells. MiR-760-regulated cell properties through reduction of BST2. In addition, miR-760 inhibited tumourigenesis in a nude mouse xenograft model in vivo. In conclusion, our results demonstrated that miR-760 exhibited a suppressive role in gastric cancer via inhibiting BST2, indicating that miR-760/BST2 axis may provide promising therapeutic target for gastric cancer.
Insights
MicroRNA-760 (miR-760) is down-regulated in gastric cancer. Restoring miR-760 suppresses tumor growth and invasion by inhibiting BST2, offering a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Gastric cancer is a major global health threat.
- MicroRNA-760 (miR-760) is significantly down-regulated in advanced gastric cancer.
- The precise role of miR-760 in gastric cancer remains to be fully elucidated.
Purpose of the Study:
- To investigate the role of miR-760 in gastric cancer.
- To identify the molecular mechanisms underlying miR-760's function.
- To explore the therapeutic potential of targeting the miR-760/BST2 axis.
Main Methods:
- Analysis of miR-760 expression in gastric cancer tissues.
- In vitro studies involving miR-760 overexpression and knockdown in gastric cancer cell lines (MGC-803, SGC-7901).
- In vivo tumor xenograft model in nude mice.
Main Results:
- miR-760 was confirmed to be down-regulated in gastric cancer.
- miR-760 overexpression suppressed cell viability, proliferation, migration, and invasion, while promoting apoptosis and inhibiting MMP activity.
- Bone marrow stromal antigen 2 (BST2) was identified as a direct target of miR-760, with miR-760 negatively regulating BST2 expression.
- miR-760 inhibited tumor growth in vivo.
Conclusions:
- miR-760 acts as a tumor suppressor in gastric cancer.
- The suppressive role of miR-760 is mediated through the inhibition of BST2.
- The miR-760/BST2 axis represents a promising therapeutic target for gastric cancer treatment.
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