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Dlgap1 negatively regulates browning of white fat cells through effects on cell proliferation and apoptosis

Ju Zhang1,2, Jie Yang1,2, Nan Yang1,2

  • 1State Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, Hohhot, 010070, China.

Lipids in Health and Disease
|March 15, 2020
PubMed
Abstract

Insights

Discs, Large (Drosophila) Homolog-Associated Protein 1 (Dlgap1) negatively impacts white fat browning. Manipulating Dlgap1 levels influences adipocyte proliferation and apoptosis, offering potential therapeutic insights for obesity.

Area of Science:

  • Cell Biology
  • Metabolic Research
  • Adipocyte Biology

Background:

  • Obesity is linked to white fat accumulation.
  • White fat browning is a strategy to combat obesity.
  • The role of Dlgap1 in adipocyte browning was previously unknown.

Purpose of the Study:

  • To investigate the function of Dlgap1 in white and brown adipocytes.
  • To determine Dlgap1's effect on adipocyte browning and related processes.

Main Methods:

  • Primary cell culture of white and brown adipocytes.
  • Gene manipulation techniques: overexpression and knockout of Dlgap1.
  • Analysis of gene expression, cell proliferation, apoptosis, and maturation.

Main Results:

  • Dlgap1 overexpression in brown adipocytes suppressed browning markers and increased proliferation/apoptosis.
  • Dlgap1 knockout in white adipocytes promoted browning markers and increased maturation.
  • Dlgap1 manipulation affected adipocyte proliferation and apoptosis but not brown adipocyte maturation.

Conclusions:

  • Dlgap1 acts as a negative regulator of white adipocyte browning.
  • Dlgap1 influences adipocyte proliferation and apoptosis, impacting metabolic health.
  • Targeting Dlgap1 may offer a novel therapeutic approach for obesity.
Keywords:
ApoptosisBrown fatDlgap1ProliferationWhite fat

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