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Oxidative stress response in reference and clinical Staphylococcus aureus strains under Linezolid exposure

Sol Romina Martínez1, Virginia Aiassa2, Claudia Sola3

  • 1InstitutoMultidisciplinario de Biología Vegetal (IMBIV), CONICET and Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, X5000HUA Córdoba, Argentina.

Journal of Global Antimicrobial Resistance
|March 15, 2020
PubMed
Abstract

Insights

Linezolid (LZD) treatment of Methicillin-resistant Staphylococcus aureus (MRSA) strains resulted in a slight increase in reactive species, but did not cause significant oxidative stress. This study shows negligible oxidative stress in MRSA exposed to LZD.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Oxidative Stress

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) is a prevalent pathogen with multi-drug resistance.
  • The impact of antimicrobial agents on bacterial oxidative stress is not fully understood.

Purpose of the Study:

  • To investigate the effect of linezolid (LZD) on oxidative stress markers in Staphylococcus aureus strains.
  • To assess the role of oxidative stress in bacteria exposed to bacteriostatic antimicrobial agents.

Main Methods:

  • Measured oxidative stress markers including superoxide dismutase (SOD) activity, global antioxidant response, advanced oxidation protein products (AOPP), and glutathione levels.
  • Utilized 28 clinical and 2 reference strains of S. aureus.
  • Exposed strains to linezolid (LZD) and compared results to untreated controls.

Main Results:

  • 10 out of 30 strains showed a 22-56% increase in reactive species under LZD treatment.
  • Clinical strains exhibited higher reactive species generation than reference strains.
  • Antioxidant response and oxidized protein levels remained largely unaffected, indicating no acute oxidative stress.

Conclusions:

  • Linezolid (LZD) exposure induces a minor increase in reactive species in S. aureus but does not lead to significant oxidative stress.
  • This is the first study to report the oxidative stress behavior of clinical and reference S. aureus strains exposed to LZD.
  • The findings suggest that LZD does not cause acute oxidative stress in S. aureus.
Keywords:
Clinical strainsMethicillin-resistant Staphylococcus aureusOxazolidinoneOxidative stress

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