Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

3.4K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
3.4K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

953
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
953
Ion Exchange01:17

Ion Exchange

1.1K
Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
1.1K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

763
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
763
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

7.1K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
7.1K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

1.6K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Polypharmacy and hyperpolypharmacy in elderly (≥ 65 years) pacemaker recipients: Prevalence and association with frailty, physical activity, adherence, and healthcare utilization in a prospective single-center study.

International journal of clinical pharmacology and therapeutics·2026
Same author

Side-On Cannulation Angioplasty for Late Left Main Stent Failure After Chimney Stenting in Valve-in-Valve TAVR.

JACC. Cardiovascular interventions·2026
Same author

CDHR5 splice isoform cooperation promotes apical targeting of the brush border cadherin.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2025
Same author

Synchronous Primary Lung Cancer: An Unusual Triple Presentation.

Cureus·2025
Same author

Establishing a left bundle branch area pacing program: Results from a high-volume pacing center.

Revista portuguesa de cardiologia : orgao oficial da Sociedade Portuguesa de Cardiologia = Portuguese journal of cardiology : an official journal of the Portuguese Society of Cardiology·2025
Same author

Primary Stability of Zirconia Dental Implants with Cylindrical and Tapered Designs Across Varying Bone Densities: An In Vitro Evaluation.

Dentistry journal·2024

Related Experiment Video

Updated: Dec 26, 2025

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.4K

Routine, ensemble characterisation of electrophoretic mobility in high and saturated ionic dispersions.

Jake Austin1, Diogo Fernandes1, Matthew J A Ruszala1

  • 1Nanotechnology group of Malvern Panalytical Ltd., Grovewood Road, Malvern, WR14 1XZ, United Kingdom.

Scientific Reports
|March 15, 2020
PubMed
Summary

A novel micro-electrophoresis technique with a diffusion barrier enables accurate nanoparticle characterization. This method overcomes limitations of previous approaches, allowing for reliable electro-kinetic measurements in various conditions.

More Related Videos

Adapting Taylor Dispersion to Measure the Dispersion Coefficient of Electrolyte Solutions via an Accessible Microfluidic Setup
09:56

Adapting Taylor Dispersion to Measure the Dispersion Coefficient of Electrolyte Solutions via an Accessible Microfluidic Setup

Published on: October 7, 2025

442
Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

8.3K

Related Experiment Videos

Last Updated: Dec 26, 2025

Assembly and Characterization of Polyelectrolyte Complex Micelles
08:44

Assembly and Characterization of Polyelectrolyte Complex Micelles

Published on: March 2, 2020

11.4K
Adapting Taylor Dispersion to Measure the Dispersion Coefficient of Electrolyte Solutions via an Accessible Microfluidic Setup
09:56

Adapting Taylor Dispersion to Measure the Dispersion Coefficient of Electrolyte Solutions via an Accessible Microfluidic Setup

Published on: October 7, 2025

442
Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

8.3K

Area of Science:

  • Colloid and Surface Science
  • Nanotechnology
  • Electrochemistry

Background:

  • Industrialization of nanoparticle manufacturing leads to environmental release.
  • Characterization of nano-pharmaceuticals and biological systems requires in vivo conditions.
  • Existing electro-kinetic models need experimental validation.

Purpose of the Study:

  • To introduce a simple and effective experimental tool for nanoparticle characterization.
  • To validate theoretical models of electro-kinetic behavior.
  • To explore new nano-systems and their properties.

Main Methods:

  • Micro-electrophoresis with a diffusion barrier to isolate dispersed phases from electrode surfaces.
  • Measurements conducted in standard laboratory light scattering instruments.
  • Short measurement durations (a few minutes).

Main Results:

  • Successfully prevented outgassing, precipitation, and sample degradation.
  • Reproduced theoretically predicted phenomena: asymptotic, non-zero electrophoretic mobility with increasing ionic strength.
  • Observed cationic Hofmeister series dependency, charge inversion, and decreasing mobility with increasing molarity and pH.

Conclusions:

  • The diffusion barrier micro-electrophoresis technique is effective for nanoparticle characterization.
  • The method validates theoretical models and enables exploration of new nano-systems.
  • Standard operating procedures are provided to facilitate further research.