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Impulse cytophotometric DNA analysis in pituitary adenomas
A Ahyai1, A Hori, V Bockermann
1Department of Neurosurgery, University of Göttingen, West Germany.
Neurosurgical Review
|January 1, 1988
Summary
Flow cytometry revealed three pituitary adenoma proliferation groups. High proliferation (PI > 15) indicated non-benign tumors, but hormone activity did not correlate with DNA ploidy.
Area of Science:
- Endocrinology
- Oncology
- Molecular Biology
Background:
- Pituitary adenomas are common tumors, and their biological behavior varies.
- Accurate assessment of tumor proliferation is crucial for diagnosis and treatment planning.
Purpose of the Study:
- To classify pituitary adenomas based on DNA content and proliferation index using flow cytometry.
- To correlate flow cytometric findings with histological characteristics and clinical behavior.
Main Methods:
- Flow cytometric DNA analysis was performed on 32 surgically removed pituitary adenomas.
- Tumor cell DNA histograms were compared between fresh tissue and cultured cells.
- Proliferation index (PI) was calculated and tumors were grouped accordingly.
Main Results:
- The majority of pituitary adenomas exhibited slow proliferation (PI < 10).
- A subset of tumors showed higher proliferation indices (PI 10-15).
- Tumors with PI > 15 displayed histological features of malignancy, including increased chromatin, nuclear polymorphism, mitoses, and invasion, indicating non-benign status.
Conclusions:
- Flow cytometry is a valuable tool for assessing pituitary adenoma proliferation.
- High proliferation index (PI > 15) is associated with aggressive tumor behavior.
- No direct correlation was found between hormone secretory activity and DNA ploidy in these tumors.