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Updated: Dec 26, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
Published on: April 11, 2019
CRISPR-Cas12b enables efficient plant genome engineering
Meiling Ming1,2, Qiurong Ren3, Changtian Pan1
1Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.
Abstract:
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas12b is a newly emerged genome engineering system. Here, we compared Cas12b from Alicyclobacillus acidoterrestris (Aac), Alicyclobacillus acidiphilus (Aa), Bacillus thermoamylovorans (Bth) and Bacillus hisashii (Bh) for genome engineering in rice, an important crop. We found AaCas12b was more efficient than AacCas12b and BthCas12b for targeted mutagenesis, which was further demonstrated in multiplexed genome editing. We also engineered the Cas12b systems for targeted transcriptional repression and activation. Our work establishes Cas12b as the third promising CRISPR system, after Cas9 and Cas12a, for plant genome engineering.
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