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Updated: Dec 26, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

CRISPR-Cas12b enables efficient plant genome engineering.

Meiling Ming^1,2, Qiurong Ren³, Changtian Pan¹

¹Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, USA.

|March 15, 2020

Summary

The study highlights the efficiency of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas12b for genome engineering in rice. Alicyclobacillus acidiphilus Cas12b (AaCas12b) demonstrated superior performance in targeted mutagenesis and multiplexed editing.

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Area of Science:

Plant biotechnology
Molecular biology
Genome editing

Background:

CRISPR-Cas12b is an emerging genome engineering tool.
Its efficiency across different bacterial strains for crop improvement is not well-established.

Purpose of the Study:

To compare the efficacy of different Cas12b variants from various bacterial species for genome engineering in rice.
To evaluate the potential of Cas12b systems for gene transcriptional regulation in plants.

Main Methods:

Comparative analysis of Cas12b variants (Aac, Aa, Bth, Bh) in rice.
Assessment of targeted mutagenesis and multiplexed genome editing capabilities.
Engineering Cas12b for transcriptional repression and activation.

Main Results:

Alicyclobacillus acidiphilus Cas12b (AaCas12b) showed higher efficiency in targeted mutagenesis compared to AacCas12b and BthCas12b.
AaCas12b was effective in multiplexed genome editing applications.
Cas12b systems were successfully engineered for transcriptional repression and activation in rice.

Conclusions:

Cas12b represents a promising third-generation CRISPR system for plant genome engineering.
AaCas12b is a highly efficient variant for diverse genome editing applications in rice.
The engineered Cas12b systems offer new avenues for precise gene regulation in crops.