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Published on: March 21, 2018
An electrochemiluminescence aptasensor based on Ru(bpy)32+ encapsulated titanium-MIL-125 metal-organic framework for
Jingxian Li1, Xueling Shan1,2, Ding Jiang1,2
1Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, China.
Abstract:
Ultrasensitive and selective determination of bisphenol A (BPA) was achieved by a solid-state electrochemiluminescence (ECL) aptasensor. The Ti-based MOFs (MIL-125, MIL stands for Materials of Institute Lavoisier) have porous structure and large specific surface area. They were used as a carrier for Ru(bpy)32+ by a hydrothermal synthesis method. The Ru(bpy)32+ encapsulated MIL-125 (Ru(bpy)32+@MIL-125) was dropped on a glass carbon electrode (GCE) and used as working electrode. After that, the thiol-based aptamer of BPA was used to modify the working electrode by Ti-S bond. As a result of the specific binding affinity between aptamer and BPA, the ECL signal was significantly quenched. Thus, a selective electrochemiluminescence (ECL) aptasensor for BPA was developed. The ECL aptasensor based on Ru(bpy)32+@MIL-125 has a good ECL response toward detecting BPA. Under optimal conditions, the aptasensor can detect BPA in a wide linear range from 1.0 × 10-12 to 1.0 × 10-6 M with an excellent determination limit of 6.1 × 10-13 M (S/N = 3). The ECL aptasensor can selectively detect BPA in a mixture containing BPA interference compounds.
