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Updated: Dec 26, 2025

Influenza A Virus Studies in a Mouse Model of Infection
Published on: September 7, 2017
Detection of Influenza A Antibodies in Avian Samples by ELISA
Erica Spackman1, Mary Lea Killian2
1Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research Center, U.S. Department of Agriculture, Agricultural Research Service, Athens, GA, USA. Erica.spackman@usda.gov.
Abstract:
ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive than in-house tests, they provide a ready-to-use assay with good quality control. Numerous sample types can be processed for ELISA: serum, plasma, egg yolk, or blood collected on filter paper. High-quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, result interpretation, and troubleshooting are discussed.

