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Development of dual-tool nanosensor for cysteine based on N-(1-naphthyl)ethylenediamine cation functionalized silver
Shamim Ahmed Khan1, Rupasree Choudhury1, Moumita Majumdar1
1Department of Chemistry, National Institute of Technology Agartala, Agartala, Tripura 799046, India.
Summary
This study introduces a novel dual-tool nanosensor using N-(1-naphthyl)ethylenediamine cation (NEDA+) stabilized silver nanoparticles (NEDA-AgNPs) for detecting cysteine. The NEDA-AgNPs demonstrate effective dual-mode sensing for both anionic and neutral cysteine forms.
Area of Science:
- Nanotechnology
- Analytical Chemistry
- Biochemistry
Background:
- Silver nanoparticles (AgNPs) are widely used in nanosensor development.
- Cysteine is a crucial amino acid with implications in various biological processes.
- Developing selective and sensitive detection methods for cysteine is essential.
Purpose of the Study:
- To develop a dual-tool nanosensor for the detection of cysteine in its anionic and neutral forms.
- To utilize N-(1-naphthyl)ethylenediamine cation (NEDA+) stabilized AgNPs (NEDA-AgNPs) for enhanced stability and dual sensing capabilities.
- To establish spectrophotometric and spectrofluorimetric methods for cysteine quantification.
Main Methods:
- Synthesis of NEDA+ stabilized AgNPs (NEDA-AgNPs).
- Utilizing Surface Plasmon Resonance (SPR) for spectrophotometric detection of AgNP aggregation induced by cysteine.
- Employing fluorescence quenching and reversal of NEDA+ in NEDA-AgNPs for spectrofluorimetric detection of cysteine.
- Assessing detection limits and binding affinities for both anionic and neutral cysteine.
Main Results:
- NEDA-AgNPs exhibited dual-tool sensing capabilities for cysteine.
- Spectrophotometric and spectrofluorimetric methods achieved low detection limits for anionic cysteine (0.1784–1.598 μM and 0.0842–2.0 μM, respectively).
- Anionic cysteine showed stronger binding affinity to NEDA+ cations compared to neutral cysteine, with excellent recovery rates (>95%) from real samples.
Conclusions:
- NEDA-AgNPs serve as an effective dual-tool and dual-form nanosensor for cysteine quantification.
- The developed nanosensor offers a sensitive and selective method for analyzing cysteine in various samples.
- This system presents an attractive analytical tool for chemists and researchers.
