The challenge of intracellular temperature

This short review begins with a brief introductory summary of luminescence nanothermometry. Current applications of luminescence nanothermometry are introduced in biological contexts. Then, theoretical bases of the "temperature" that luminescence nanothermometry determines are discussed. This argument is followed by the 10⁵ gap issue between simple calculation and the measurements reported in literatures. The gap issue is challenged by recent literatures reporting single-cell thermometry using non-luminescent probes, as well as a report that determines the thermal conductivity of a single lipid bilayer using luminescence nanothermometry. In the end, we argue if we can be optimistic about the solution of the 10⁵ gap issue.