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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
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Recent advances in bioimaging with high-speed atomic force microscopy.

Takayuki Uchihashi1,2, Christian Ganser3,4

  • 1Department of Physics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi, 464-8602, Japan. uchihast@d.phys.nagoya-u.ac.jp.

Biophysical Reviews
|March 16, 2020
PubMed
Summary

High-speed atomic force microscopy (HS-AFM) enables label-free visualization of protein dynamics and interactions in liquid. This review highlights recent bioimaging and mechanical property applications of HS-AFM in biophysical research.

Keywords:
Conformational dynamicsHigh-speed atomic force microscopyIntermolecular interactionMechanical indentationSingle-molecule imaging

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Area of Science:

  • Biophysics
  • Microscopy
  • Protein Science

Background:

  • High-speed atomic force microscopy (HS-AFM) offers label-free visualization of biomolecules in liquid.
  • It allows direct observation of protein structural changes and molecular interactions.

Purpose of the Study:

  • To review recent bioimaging applications of HS-AFM.
  • To explore novel applications leveraging AFM's mechanical property measurements.

Main Methods:

  • High-speed atomic force microscopy (HS-AFM) for dynamic protein analysis.
  • Label-free imaging in aqueous environments.
  • AFM-based mechanical property measurements.

Main Results:

  • HS-AFM has been applied to diverse proteins: motor, membrane, DNA-binding, amyloid, and artificial proteins.
  • Recent studies showcase advanced bioimaging capabilities.
  • Novel applications integrate structural and mechanical data acquisition.

Conclusions:

  • HS-AFM is an indispensable tool for biophysical research.
  • Its versatility extends to analyzing protein dynamics, interactions, and mechanical characteristics.
  • Future research can benefit from combined structural and mechanical insights.