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Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

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Report on fluorescence lifetime imaging using multiphoton laser scanning microscopy targeting sentinel lymph node

Jeemol James¹, Despoina Kantere², Jonas Enger³

¹University of Gothenburg, Biomedical Photonics Group, Department of Chemistry and Molecular Biology,, Sweden.

Journal of Biomedical Optics

|March 16, 2020

Summary

Multiphoton laser scanning microscopy with fluorescence lifetime analysis (MPM-FLIM) shows promise for rapid sentinel lymph node biopsy diagnostics in melanoma patients. This optical technology enhances morphological contrast for improved metastasis detection.

Keywords:

autofluorescence fluorescence lifetime imaging malignant melanoma multiphoton microscopy sentinel lymph node

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Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

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Published on: February 9, 2012

Area of Science:

Oncology
Medical Imaging
Biophotonics

Background:

Sentinel lymph node (SLN) biopsy is crucial for metastasis staging in melanoma.
Prompt histopathological analysis aids diagnostics, driving exploration of optical technologies.

Purpose of the Study:

To investigate multiphoton laser scanning microscopy (MPM) combined with fluorescence lifetime analysis (FLIM) for ex vivo lymph node (LN) tissue examination.
To assess the potential of MPM-FLIM for enhancing diagnostic capabilities in SLN analysis.

Main Methods:

MPM-FLIM was applied to five deparaffinized human LN tissue samples (three metastasis-positive, two negative) from melanoma patients.
An experimental MPM setup with a time-correlated single photon counting module was utilized for FLIM acquisition.

Main Results:

MPM imaging revealed morphological features comparable to conventional histology.
FLIM analysis demonstrated enhanced morphological contrast, differentiating between metastatic cells, lymphocytes, and erythrocytes.

Conclusions:

MPM-FLIM shows significant promise for application in SLN diagnostics.
Further translational studies using fresh tissue samples are encouraged to validate these findings.