Purification, structural characterization, and antioxidant activity of the COP-W1 polysaccharide from Codonopsis...

A new polysaccharide named COP-W1 was extracted from the root of Codonopsis tangshen Oliv using hot water extraction and ethanol precipitation. COP-W1, with a molecular weight of 2.34 × 10⁴ Da, was purified using gel permeation chromatography. The monosaccharide composition of COP-W1 was mannose, rhamnose, glucose, and galactose, and its molar ratio was 20.32:1:1.27:36.13. Structural characterization of COP-W1 was confirmed by Fourier transform infrared spectrometry (FT-IR), gas chromatograph-mass spectrometry (GC-MS), and nuclear magnetic resonance (NMR) analysis. The results indicated that the backbone of COP-W1 was composed of (1→3)-linked-galactose, (1→3, 6)-linked-mannose, (1→4)-linked-mannose, and (1→6)-linked-mannose residues, which branched at O-3. The branches consisted of (1→2, 6)-linked-galactose, (1→6)-linked-galactose, and (1→4)-linked-galactose, each terminating with α-galactose. In addition, antioxidant assays demonstrated that COP-W1 has strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (half maximal inhibition concentration, IC₅₀, at 0.610 mg/mL), and suggested that COP-W1 has the potential to be used as a natural antioxidant.