Arsenic species in electronic cigarettes: Determination and potential health risk

Human exposure to contaminants from electronic cigarettes (e-cigarettes) and the associated health effects are poorly understood. There has been no report on the speciation of arsenic in e-liquid (solution used for e-cigarettes) and aerosols. We report here determination of arsenic species in e-liquids and aerosols generated from vaping the e-liquid. Seventeen e-liquid samples of major brands, purchased from local and online stores in Canada and China, were analyzed for arsenic species using high-performance liquid chromatography and inductively coupled plasma mass spectrometry. Aerosols condensed from vaping the e-liquids were also analyzed and compared for arsenic species. Six arsenic species were detected, including inorganic arsenate (iAs^V), arsenite (iAs^III), monomethylarsonic acid (MMA), and three new arsenic species not reported previously. In e-liquids, iAs^III was detected in 59%, iAs^V in 94%, and MMA in 47% of the samples. In the condensate of aerosols from vaping the e-liquids, iAs^III was detected in 100%, iAs^V in 88%, and MMA in 13% of the samples. Inorganic arsenic species were predominant in e-liquids and aerosols of e-cigarettes. The concentration of iAs^III in the condensate of aerosols (median 3.27 μg/kg) was significantly higher than that in the e-liquid (median 1.08 μg/kg) samples. The concentration of inorganic arsenic in the vaping air was approximately 3.4 μg/m³, which approaches to the permissible exposure limit (10 μg/m³) set by the United States Occupational Safety and Health Administration (OSHA). According to the Environmental Protection Agency's unit risk factor (4.3 × 10^-3 per μg/m³) for inhalation exposure to inorganic arsenic in the air, the estimated excess lung cancer risk from lifetime exposure to inorganic arsenic in the e-cigarette vaping air (3.4 μg/m³), assuming e-cigarette vaping at 1% of the time, is as high as 1.5 × 10^-4. These results raise health concerns over the exposure to arsenic from electronic cigarettes.