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Related Experiment Video

Updated: Dec 26, 2025

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery

Published on: October 27, 2023

A Method to Analyze Urinary Extracellular Vesicles.

Piyawan Paisrisarn¹, Takao Yasui^1,2,3, Yoshinobu Baba^1,3,4

¹Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|March 17, 2020

Summary

This summary is machine-generated.

Urinary extracellular vesicles (UEVs) are valuable biomarkers for various diseases. Standardizing UEV analysis methods is crucial for reliable clinical applications and accurate disease diagnosis.

Keywords:

Urinary extracellular vesicles analytical method biomarkers cancer characterization isolation qualitative analysis quantitative analysis

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Characterizing Extracellular Vesicles from Biological Fluids

Characterizing Extracellular Vesicles from Biological Fluids

Published on: February 28, 2025

Area of Science:

Biochemistry
Cell Biology
Clinical Diagnostics

Background:

Extracellular vesicles (EVs) mediate cell-to-cell communication via molecular cargo.
Urine is an accessible biofluid for collecting EVs, termed urinary extracellular vesicles (UEVs).
UEVs show promise as biomarkers for urological and non-urological conditions.

Purpose of the Study:

To review current techniques for analyzing UEVs.
To present commonly used, novel, and emerging UEV analysis methods.
To guide the selection of appropriate UEV analysis techniques.

Main Methods:

Review of existing literature on UEV analysis protocols.
Summary of workflow procedures from sampling to analysis.
Evaluation of advantages and disadvantages of various methodologies.

Main Results:

High variability and low reproducibility challenge UEV analysis standardization.
Commonly used, newly developed, and potential future UEV techniques are discussed.
Workflow steps and their associated pros and cons are outlined.

Conclusions:

Standardized protocols are essential for reproducible UEV analysis.
Careful consideration of method advantages and disadvantages is needed for UEV biomarker development.
Selecting suitable UEV analysis methods is key for clinical applications.