[Interaction of androgen-receptor complexes in rat liver cytosol with cellular nuclei and DNA]

A study was made of the ability of androgen-receptor complexes (ARC) of male rat liver cytosol to interact with hepatic cell nuclei and DNA in a model free-cell system and after administration of androgens to animals. In a free-cell system androgen receptors (AR) within a partially purified cytosol preparation were capable of binding strongly with hepatic cell nuclei and DNA of cellulose-conjugated cow spleen. The administration of testosterone propionate for 3 days resulted in the redistribution of liver AR between cytoplasm and nuclei during testing 1 h after the last injection. The observed regularities were unrelated to changes in AR content caused by the regulatory effect of administered androgen. Similar changes in AR content in the studied subcellular fractions were revealed 1 h after single administration of R1881 but not testosterone propionate. The revealed regularities were indicative of a possibility of AR involvement in androgenic action on the liver at the nuclear level.