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Updated: Dec 26, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
Long noncoding RNAs expression profile and long noncoding RNA-mediated competing endogenous RNA network in
Hao Bo1,2,3, Zhizhong Liu1,2, Fang Zhu2
1Department of Urology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan Cancer Hospital, Changsha, PR China.
Abstract:
Aim: To analyze the expression profile and competing endogenous RNA (ceRNA) network of long noncoding RNAs (lncRNAs) in nonobstructive azoospermia (NOA). Materials & methods: The lncRNA expression profile in NOA was determined by microarray reanalysis. Differential expression analysis was performed by R software. The ceRNA network was constructed using correlation analysis and gene target miRNA prediction. Metascape was used for enrichment analysis. Again ceRNA network was validated by quantitative real-time PCR. Results: Many lncRNAs are differently expressed in NOA. LncRNAs might participate in spermatogenesis through ceRNA mechanism. The ceRNA network included male gamete generation and other pathways. LINC00467 in the network regulated the expression of LRGUK and TDRD6. Conclusion: LncRNAs are involved in NOA and potential biomarkers and therapeutic targets for NOA.
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