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Related Experiment Video

Updated: Dec 26, 2025

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

A simple and efficient method for generating human retinal organoids.

Florian Regent¹, Holly Y Chen¹, Ryan A Kelley¹

¹Neurobiology, Neurodegeneration & Repair Laboratory, National Eye Institute, National Institutes of Health, Bethesda, MD.

Molecular Vision

|March 17, 2020

Summary

This summary is machine-generated.

Researchers developed a simpler method to generate retinal organoids (ROs) from stem cells. This technique bypasses complex dissection, enabling easier, large-scale production for disease modeling and therapies.

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Last Updated: Dec 26, 2025

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Generation of Retinal Organoids from Healthy and Retinal Disease-Specific Human-Induced Pluripotent Stem Cells

Published on: December 9, 2022

Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

Retinal Organoid Induction System for Derivation of 3D Retinal Tissues from Human Pluripotent Stem Cells

Published on: April 12, 2021

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Directed Induction of Retinal Organoids from Human Pluripotent Stem Cells

Published on: April 21, 2021

Area of Science:

Stem cell biology
Developmental biology
Ophthalmology

Background:

Human pluripotent stem cells generate retinal organoids (ROs) that mimic in vivo retinal development.
ROs are valuable for studying retinogenesis, modeling retinal diseases, and developing therapies.
Current RO generation protocols are complex, labor-intensive, and hinder large-scale production.

Purpose of the Study:

To develop a simplified and efficient method for generating retinal organoids.
To overcome the limitations of current labor-intensive dissection techniques.
To facilitate large-scale production of retinal organoids for research and therapeutic applications.

Main Methods:

A novel protocol involving scraping entire adherent cultures and transitioning to free-floating conditions was employed.
Cell aggregates were cultured in 3D to promote retinal organoid formation.
The efficiency and yield of retinal organoid generation were assessed.

Main Results:

A simple scraping method efficiently generates retinal organoids (ROs).
Optic vesicles formed readily within 1–7 days, simplifying harvesting.
The 2D to 3D culture transition increased RO yield and association with pigment epithelium.

Conclusions:

This robust and efficient protocol simplifies retinal organoid generation.
The method facilitates large-scale production of ROs from pluripotent stem cells.
The improved protocol supports advancements in human disease modeling and therapy development.