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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Exon Recombination02:32

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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Updated: Dec 26, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
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Alternative splicing signatures in preimplantation embryo development.

Geng G Tian1, Jing Li1,2, Ji Wu1,3,4

  • 11Renji Hospital, Key Laboratory for the Genetics of Developmental & Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200240 China.

Cell & Bioscience
|March 17, 2020
PubMed
Summary

Alternative splicing (AS) is vital for embryo development, with the two-cell stage being critical for gene transcription. AS genes are enriched at TAD boundaries, influencing chromatin structure during early development.

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Area of Science:

  • Developmental Biology
  • Genomics
  • Epigenetics

Background:

  • Preimplantation embryo development requires precise temporal and spatial gene expression control.
  • Alternative splicing (AS) is essential for regulating gene expression by modifying genomic instructions into functional proteins.
  • Studying AS enhances understanding of transcription and splicing in early embryonic development.

Purpose of the Study:

  • To investigate the role and signatures of alternative splicing during preimplantation embryo development.
  • To identify critical transcriptional stages in early embryonic development.
  • To explore the relationship between alternative splicing, gene transcription, and chromatin structure.

Main Methods:

  • Analysis of single-cell RNA sequencing (scRNA-seq) data to identify key transcription stages.
  • Examination of alternative splicing patterns across preimplantation development.
  • Integration of high-throughput chromosome conformation (Hi-C) data to assess chromatin organization.

Main Results:

  • The two-cell stage was identified as a critical period for gene transcription in preimplantation embryos.
  • Alternative splicing is widespread during preimplantation development, particularly at the two-cell stage.
  • Alternative splicing genes are significantly enriched at topologically associating domain (TAD) boundaries, independent of A/B compartments.

Conclusions:

  • This study provides novel insights into the interplay between alternative splicing, gene transcription, and chromatin structure.
  • Findings highlight the significance of the two-cell stage and TAD boundaries in regulating gene expression during early development.