Polymer compaction and bridging-induced clustering of protein-inspired patchy particles

There are many proteins or protein complexes which have multiple DNA binding domains. This allows them to bind to multiple points on a DNA molecule (or chromatin fibre) at the same time. There are also many proteins which have been found to be able to compact DNAin vitro, and many others have been observed in foci or puncta when fluorescently labelled and imagedin vivo. In this work we study, using coarse-grained Langevin dynamics simulations, the compaction of polymers by simple model proteins and a phenomenon known as the 'bridging-induced attraction'. The latter is a mechanism observed in previous simulations [Brackleyet al2013Proc. Natl Acad. Sci. USA110E3605], where proteins modelled as spheres form clusters via their multivalent interactions with a polymer, even in the absence of any explicit protein-protein attractive interactions. Here we extend this concept to consider more detailed model proteins, represented as simple 'patchy particles' interacting with a semi-flexible bead-and-spring polymer. We find that both the compacting ability and the effect of the bridging-induced attraction depend on the valence of the model proteins. These effects also depend on the shape of the protein, which determines its ability to form bridges.