Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

62.0K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.0K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

14.1K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
14.1K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

4.1K
4.1K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.0K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

9.9K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.9K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.2K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutations and structural variants arising during double-strand break repair.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Prime Editing for p47<sup>phox</sup>-Deficient Chronic Granulomatous Disease.

The New England journal of medicine·2025
Same author

Proteomic sensors for quantitative multiplexed and spatial monitoring of kinase signaling.

Nature communications·2025
Same author

Learning about break-induced replication from bacteriophages.

Nature reviews. Molecular cell biology·2025
Same author

Arp2/3 and type-I myosins control chromosome mobility and end-resection at double-strand breaks in S. cerevisiae.

Nature communications·2025
Same author

sPLA2-IIA modifies progranulin deficiency phenotypes in mouse models.

Molecular neurodegeneration·2025

Related Experiment Video

Updated: Dec 26, 2025

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.5K

Checkpoint Responses to DNA Double-Strand Breaks.

David P Waterman1, James E Haber1, Marcus B Smolka2

  • 1Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts 02454, USA;

Annual Review of Biochemistry
|March 17, 2020
PubMed
Summary

Cells activate a DNA damage checkpoint (DDC) to repair DNA double-strand breaks (DSBs). This response prevents cell death and cancer by halting the cell cycle and initiating DNA repair, studied in yeast and mammals.

Keywords:
DNA double-strand breakDNA repaircell cyclecheckpointkinases

More Related Videos

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.9K
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.5K

Related Experiment Videos

Last Updated: Dec 26, 2025

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
08:31

Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy

Published on: June 8, 2018

9.5K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.9K
Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.5K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Cells encounter DNA damage, including DNA double-strand breaks (DSBs), during each cell cycle.
  • Unrepaired or improperly repaired DSBs can lead to cell lethality or cancer.
  • The DNA damage checkpoint (DDC) response is a critical cellular mechanism to manage DSBs.

Purpose of the Study:

  • To examine the DNA damage checkpoint (DDC) response induced by DNA double-strand breaks (DSBs).
  • To investigate the conserved mechanisms of DDC in both budding yeast and mammalian systems.

Main Methods:

  • Comparative analysis of DDC pathways.
  • Utilizing budding yeast as a model organism.
  • Investigating DDC in mammalian cells.

Main Results:

  • The DDC response involves cell cycle arrest, gene expression reprogramming, and recruitment of DNA repair factors.
  • Conserved elements of the DDC response exist between yeast and mammals.
  • The DDC is essential for preventing the inheritance of damaged chromosomes.

Conclusions:

  • The DNA damage checkpoint is a fundamental cellular process for maintaining genomic integrity.
  • Understanding DDC mechanisms in model systems like yeast can provide insights into human disease, including cancer.
  • The study highlights the conserved nature of DNA damage response pathways across eukaryotes.