Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mesenchymal Stromal Cells Improve Islet β-Cell Functional Survival: Analysis of Extracellular Vesicle-Trafficked Proteins and miRNAs.

Cells·2026
Same author

NAMPT activity plays a key role in driving autoimmune processes that mediate beta-cell death and type 1 diabetes development in mice.

Cell death & disease·2026
Same author

Impaired glycosylation promotes rapid transition to hepatocellular carcinoma in model of diet-induced steatotic liver disease.

The Journal of clinical investigation·2026
Same author

Role of collagen type III/ITGB1 signalling in stellate cell-mediated regulation of β-cell survival and insulin secretion in the pancreatic islet microenvironment.

Diabetic medicine : a journal of the British Diabetic Association·2026
Same author

Hologenomic analysis of rectal mucus sampling for detection of adenomatous polyps and colorectal cancer.

Nature communications·2025
Same author

Localized GLP1 receptor pre-internalization directs pancreatic alpha cell to beta cell communication.

Cell metabolism·2025

Related Experiment Video

Updated: Dec 26, 2025

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

9.9K

Assessing Mouse Islet Function.

Patricio Atanes1, Inmaculada Ruz-Maldonado1, Oladapo E Olaniru1

  • 1Department of Diabetes, School of Life Course Sciences, King's College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 18, 2020
PubMed
Summary

Researchers detail methods for isolating mouse islets of Langerhans from pancreatic tissue. These protocols enable in vitro functional characterization, including assessments of viability, hormone secretion, and beta-cell proliferation.

Keywords:
Islet hormone secretionIslet viability assaysIslets of LangerhansMouse islet isolationSecond messengersβ-cell

More Related Videos

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

66.0K
Glucose-Stimulated Insulin Secretion via Perfusion through the Mice Vasculature with an Intact Pancreas
04:41

Glucose-Stimulated Insulin Secretion via Perfusion through the Mice Vasculature with an Intact Pancreas

Published on: July 25, 2025

763

Related Experiment Videos

Last Updated: Dec 26, 2025

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
10:09

Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets

Published on: May 11, 2015

9.9K
A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
12:33

A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination

Published on: June 25, 2014

66.0K
Glucose-Stimulated Insulin Secretion via Perfusion through the Mice Vasculature with an Intact Pancreas
04:41

Glucose-Stimulated Insulin Secretion via Perfusion through the Mice Vasculature with an Intact Pancreas

Published on: July 25, 2025

763

Area of Science:

  • Endocrinology
  • Cell Biology
  • Pancreatic Biology

Background:

  • Islets of Langerhans are crucial endocrine cell clusters within the pancreas.
  • Their small proportion necessitates specialized isolation techniques from exocrine tissue.
  • Understanding islet function is vital for metabolic research.

Purpose of the Study:

  • To provide detailed protocols for isolating mouse islets of Langerhans.
  • To outline methods for in vitro functional characterization of isolated islets.
  • To facilitate research on islet cell biology and function.

Main Methods:

  • Pancreatic tissue digestion to separate islets from acinar cells.
  • Islet purification techniques.
  • In vitro functional assays including viability, hormone content/secretion, second messenger generation, and beta-cell proliferation assessments.

Main Results:

  • Established protocols for successful isolation of functional mouse islets.
  • Demonstrated methods for comprehensive in vitro functional analysis.
  • Provided a framework for reproducible islet research.

Conclusions:

  • The described protocols enable robust isolation and characterization of mouse islets.
  • These methods support further investigation into islet cell physiology and pathophysiology.
  • This work serves as a valuable resource for researchers in diabetes and metabolic studies.