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Mapping the structure and biological functions within mesenchymal bodies using microfluidics
Sébastien Sart1,2, Raphaël F-X Tomasi1,2, Antoine Barizien1,2
1LadHyX and Department of Mechanics, Ecole Polytechnique, CNRS-UMR 7646, 91128 Palaiseau, France.
Science Advances
|March 18, 2020
Summary
Human mesenchymal stromal cells self-organize hierarchically in 3D organoids, revealing links between structure and molecular signaling pathways like NF-κB. This advances understanding of organoid formation for tissue repair.
Area of Science:
- Biotechnology
- Developmental Biology
- Tissue Engineering
Background:
- Organoid formation involves self-organization of cells in 3D, but underlying mechanisms remain unclear.
- Correlating cell fate with spatial organization in organoids is difficult.
Purpose of the Study:
- Investigate mechanisms of organoid formation using human mesenchymal stromal cells.
- Understand early condensation steps relevant to bone repair in vivo.
Main Methods:
- Utilized a novel microfluidics platform for organoid formation studies.
- Analyzed self-organization of heterogeneous mesenchymal stromal cells in 3D.
Main Results:
- Mesenchymal stromal cells exhibit developmentally hierarchical self-organization in 3D.
- Established a link between structural organization and local regulation of NF-κB and actin polymerization pathways.
- Demonstrated modulation of osteo-endocrine functions by these pathways.
Conclusions:
- Resolving spatial heterogeneities in cellular aggregates is crucial for linking organization and function.
- This research enhances understanding of organoid formation mechanisms for organogenesis and tissue repair.
