Related Experiment Video
Updated: Dec 26, 2025

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment
Published on: May 25, 2017
Isolation of Clostridioides difficile from different outdoor sites in the domestic environment
Sandra Janezic1, Julija Smrke2, Maja Rupnik1
1National Laboratory for Health, Environment and Food, NLZOH, Slovenia; Medical Faculty, University of Maribor, Slovenia.
Abstract:
Clostridioides difficile was isolated from 12 of 25 samples collected from the gardens of five individual houses and 132 isolates belonged to 12 PCR ribotypes. Compost material had the highest positivity rate and the highest PCR ribotype diversity in comparison to soil from vegetable and flower gardens. Isolated PCR ribotypes overlap with common human and animal types but also with divergent C. difficile lineages common for the soil environment.

