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Related Experiment Video

Updated: Dec 26, 2025

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

Transcriptomic Analysis of the Chicken MDA5 Response Genes.

Shiman Yu^1,2, Haiying Mao^1,2, Meilin Jin^1,2,3,4

¹State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

|March 19, 2020

Summary

Chicken MDA5 activates antiviral genes, with five key genes (MX1, IFI6, IFIT5, RSAD2, OASL) showing promise for inhibiting avian influenza virus replication and developing new therapies.

Keywords:

RNA-seq antiviral response chicken MDA5 interferon stimulated genes

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Last Updated: Dec 26, 2025

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

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Published on: November 1, 2014

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

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Published on: June 27, 2020

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model

Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model

Published on: July 29, 2012

Area of Science:

Immunology
Virology
Genomics

Background:

RIG-I and MDA5 are crucial pattern recognition receptors for sensing RNA viruses.
Chickens lack RIG-I but possess functional MDA5, with downstream genes mediating antiviral responses being understudied.
Understanding chicken MDA5 signaling is vital for avian antiviral strategies.

Purpose of the Study:

To investigate the transcriptional profile of chicken embryonic fibroblasts (DF1) stimulated by chicken MDA5 (chMDA5) and poly(I:C).
To identify interferon-stimulated genes (ISGs) upregulated by chMDA5 activation.
To evaluate the antiviral potential of identified ISGs against avian influenza virus H5N6.

Main Methods:

RNA sequencing (RNA-seq) to compare transcriptional profiles of DF1 cells transfected with chMDA5 and poly(I:C) against controls.
Real-time PCR to validate the upregulation of nine key ISGs.
Infection of DF1 cells with avian influenza virus H5N6 to assess ISG expression and antiviral activity.

Main Results:

chMDA5 and poly(I:C) transfection significantly induced numerous antiviral innate immune genes in DF1 cells.
Nine ISGs (MX1, IFI6, IFIT5, RSAD2, OASL, CMPK2, HELZ2, EPSTI1, OLFML1) were highly upregulated.
Avian influenza virus H5N6 infection upregulated MX1, IFI6, IFIT5, RSAD2, and OASL, whose overexpression inhibited viral replication.

Conclusions:

chMDA5 activation triggers a robust antiviral gene expression program in chickens.
Five specific ISGs demonstrate significant potential in combating avian influenza virus H5N6.
These findings offer insights for developing novel chicken antiviral therapies targeting MDA5-mediated immunity.