Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

1000
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
1000
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

404
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
404
Antibiotic Selection00:57

Antibiotic Selection

59.2K
Overview
59.2K
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

1.2K
The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA...
1.2K
Antimicrobial Proteins01:23

Antimicrobial Proteins

12.8K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
12.8K
Global Regulatory Systems01:28

Global Regulatory Systems

494
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
494

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bacterial sensitivity distributions for biocides and metals.

FEMS microbiology ecology·2026
Same author

Sewage surveillance for assessing clinical antibiotic resistance prevalence: Combining metagenomic and phenotypic data.

One health (Amsterdam, Netherlands)·2026
Same author

Author Correction: Geographics and bacterial networks differently shape the acquired and latent global sewage resistomes.

Nature communications·2026
Same author

Antibiotic surveillance: an action-oriented integrated approach.

Wellcome open research·2026
Same author

Molecular epidemiology and genetic determinants of β-lactam resistance in Pseudomonas aeruginosa isolates from patients with cystic fibrosis in Stockholm, Sweden.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same author

No Clear Evidence of Histopathological Effects Linked to NSAIDs in the Kidney or Liver of Fish Exposed to Treated Municipal Wastewaters.

Toxicologic pathology·2026

Related Experiment Video

Updated: Dec 26, 2025

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
09:37

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

9.4K

A Novel, Integron-Regulated, Class C β-Lactamase.

Maria-Elisabeth Böhm1,2, Mohammad Razavi1,2, Carl-Fredrik Flach1,2

  • 1Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden.

Antibiotics (Basel, Switzerland)
|March 19, 2020
PubMed
Summary

Two novel integron-encoded AmpC-type beta-lactamase genes, bla_IDC-1 and bla_IDC-2, were identified. These mobile genetic elements contribute to antibiotic resistance in bacteria, particularly in Asian countries.

Keywords:
Class C β-lactamaseantibiotic resistancefunctional metagenomicsintegron

More Related Videos

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

7.6K
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.0K

Related Experiment Videos

Last Updated: Dec 26, 2025

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes
09:37

Screening Foodstuffs for Class 1 Integrons and Gene Cassettes

Published on: June 19, 2015

9.4K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

7.6K
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)
08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

16.0K

Area of Science:

  • Microbiology
  • Genetics
  • Biochemistry

Background:

  • AmpC-type beta-lactamases confer resistance to a broad spectrum of beta-lactam antibiotics, posing a significant challenge in treating bacterial infections.
  • Plasmid-mediated AmpC enzymes are increasingly prevalent, often exhibiting less regulated expression compared to chromosomal versions, leading to constitutive resistance.

Purpose of the Study:

  • To identify and characterize novel AmpC-type beta-lactamase genes.
  • To investigate the mobile genetic context and potential for dissemination of these newly identified genes.

Main Methods:

  • Bioinformatic analysis of bacterial genomes and metagenomic datasets.
  • Sequence identity comparison with known beta-lactamase databases.
  • Analysis of gene location within mobile genetic elements like integrons.

Main Results:

  • Identification of two novel integron-encoded AmpC genes, bla_IDC-1 and bla_IDC-2, with <85% amino acid identity to known AmpCs.
  • These genes possess low isoelectric points, hindering differentiation by traditional methods.
  • The first evidence of ampC gene cassettes within a class 1 integron was found, indicating a mobile context.

Conclusions:

  • The discovery of integron-encoded bla_IDC-1 and bla_IDC-2 highlights a new mechanism for the spread of AmpC-mediated resistance.
  • These mobile elements have the potential for widespread dissemination in clinical settings.
  • Metagenomic analysis suggests a higher prevalence of these genes in Asian countries.