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Statistical optimization of simultaneous saccharification fermentative hydrogen production from corn stover.
Yanyan Jing1, Fang Li1, Yameng Li1
1Key Laboratory of New Materials and Facilities for Rural Renewable Energy Ministry of Agriculture, Henan Agricultural University, Zhengzhou, China.
Bioengineered
|March 19, 2020
Summary
Corn stover can be fermented by anaerobic bacteria to produce hydrogen. Optimizing substrate concentration, pH, and enzyme concentration significantly enhances hydrogen yield from corn stover saccharification and fermentation.
Area of Science:
- Biotechnology
- Renewable Energy
- Biochemical Engineering
Background:
- Corn stover is a carbohydrate-rich lignocellulosic biomass.
- Anaerobic fermentation of corn stover can yield hydrogen, a clean energy source.
- Optimizing fermentation conditions is crucial for efficient hydrogen production.
Purpose of the Study:
- To investigate and optimize influential factors for hydrogen production from corn stover saccharification and fermentation.
- To determine the interactions between factors affecting simultaneous saccharification and fermentation (SSF) of corn stover.
- To establish optimal conditions for maximizing hydrogen yield.
Main Methods:
- Utilized a response surface methodology (RSM) with a Box-Behnken Design (BBD) model.
- Studied the effects of substrate concentration, inoculation amount, pH, and enzyme concentration.
- Analyzed linear and multi-factor interactions on hydrogen production.
Main Results:
- Substrate concentration had the most significant linear influence on hydrogen production.
- The interaction between pH and enzyme concentration showed the most significant multi-factor effect.
- Optimal conditions (25 mg/mL substrate, 32.62% inoculation, pH 6.50, 172.08 mg/g enzyme) yielded 55.29 mL/g TS predicted and 56.66 mL/g TS actual hydrogen production.
Conclusions:
- Identified key factors and their interactions for optimizing corn stover-based hydrogen production.
- Provided optimal conditions for enhanced hydrogen yield through anaerobic SSF.
- Laid the foundation for industrial-scale biological hydrogen production from corn stover.
