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MicroRNA Profile Identifies miR-6165 Could Suppress Gastric Cancer Migration and Invasion by Targeting STRN4

Zhu Wang1,2, Yang Li1,2, Jian Cao1

  • 1Department of Gastroenterological Surgery, Peking University People's Hospital, Beijing 100044, People's Republic of China.

Oncotargets and Therapy
|March 19, 2020
PubMed
Abstract

Insights

MicroRNA-6165 (miR-6165) was identified as a novel player in gastric cancer (GC). This microRNA targets STRN4, suppressing GC cell migration and invasion, and shows potential for diagnosis and prognosis in GC patients.

Area of Science:

  • Molecular Oncology
  • Cancer Biology
  • Genetics

Background:

  • Aberrant microRNA (miRNA) expression is implicated in gastric cancer (GC) pathogenesis.
  • Previous studies identified differential miRNA and mRNA expression in GC tissues, necessitating identification of key molecules.
  • Understanding critical molecular players is crucial for advancing GC diagnosis and treatment.

Purpose of the Study:

  • To identify novel microRNAs (miRNAs) and their targets involved in gastric cancer (GC).
  • To elucidate the functional role of identified miRNAs in GC cell behavior.
  • To evaluate the diagnostic and prognostic potential of key molecules in GC.

Main Methods:

  • High-throughput microarray sequencing for miRNA and mRNA profiling in GC and normal tissues.
  • Bioinformatics analysis and database prediction to identify miRNA-mRNA interactions.
  • Real-time quantitative polymerase chain reaction (RT-qPCR), luciferase reporter assays, immunohistochemistry (IHC), wound healing, and Transwell assays to validate findings.
  • Statistical analysis for diagnostic and prognostic value assessment.

Main Results:

  • MicroRNA-6165 (miR-6165) was identified as a novel, differentially expressed miRNA in GC.
  • Bioinformatics and luciferase assays confirmed STRN4 as a direct target of miR-6165.
  • miR-6165 significantly suppressed gastric cancer cell migration and invasion by targeting STRN4 in vitro.
  • Both miR-6165 and STRN4 demonstrated potential diagnostic and prognostic value in GC.

Conclusions:

  • miR-6165 acts as a tumor suppressor in gastric cancer by targeting STRN4, inhibiting cell migration and invasion.
  • Further research is warranted to explore the clinical utility of miR-6165 and STRN4 as diagnostic and prognostic biomarkers for gastric cancer patients.
Keywords:
STRN4gastric cancermiR-6165migration and invasion

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