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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Related Experiment Video

Updated: Dec 26, 2025

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases
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OTUD4: A Potential Prognosis Biomarker for Multiple Human Cancers.

Xiaohui Zhao1, Xiaobo Su1, Lu Cao2

  • 1GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou 511436, People's Republic of China.

Cancer Management and Research
|March 19, 2020
PubMed
Summary

Deubiquitinase OTU domain containing 4 (OTUD4) is downregulated in many cancers, predicting poor prognosis. Overexpression of OTUD4 inhibits cancer cell growth and invasion by impacting the AKT signaling pathway, suggesting its potential as a therapeutic target.

Keywords:
OTUD4invasionmigrationprognosis biomarkerproliferation

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Deubiquitinase OTU domain containing 4 (OTUD4) is a K48-specific deubiquitinase involved in DNA damage repair.
  • The role of OTUD4 in human cancers, including its expression, prognostic significance, and biological functions, remains largely uncharacterized.

Purpose of the Study:

  • To investigate the expression level, prognostic value, and biological function of OTUD4 in various human cancers.
  • To elucidate the underlying molecular mechanisms of OTUD4 in cancer, particularly its association with the AKT signaling pathway.

Main Methods:

  • Analysis of OTUD4 mRNA expression and prognostic value using the GEPIA and TCGA databases.
  • Functional assays (MTT, Transwell, 3D culture) to assess OTUD4's role in breast, liver, and lung cancer cells.
  • Gene Set Enrichment Analysis (GSEA) to explore the correlation between OTUD4 and apoptosis and AKT signaling pathways.

Main Results:

  • OTUD4 mRNA expression was significantly downregulated across multiple human cancer types.
  • Lower OTUD4 expression correlated with poor prognosis in breast, esophageal, liver, lung, and ovarian cancers.
  • OTUD4 overexpression suppressed proliferation, migration, and invasion in breast, liver, and lung cancer cells by inhibiting the AKT signaling pathway.

Conclusions:

  • OTUD4 functions as a tumor suppressor in breast, liver, and lung cancers.
  • OTUD4 overexpression inhibits cancer progression via promoting apoptosis and suppressing the AKT pathway.
  • OTUD4 represents a potential prognostic biomarker and therapeutic target for multiple human cancers.