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Effect of New Dayuan powder on methicillin-resistant Staphylococcus aureus biofilms in vitro
1Guang'anmen Hospital, Beijing University of Chinese Medicine, Beijing 100029, China.
Objective:
To observe the effects of New Dayuan powder (NDYP) on Methicillin-resistant Staphylococcus aureus (MRSA) biofilms and the embedded bacteria in vitro.
Methods:
2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assays were used to study the effects of NDYP on developing MRSA biofilms: 100 μL of bacterial culture and 100 μL drug solution were added to wells of 96-well plates. After 24 h of incubation, the plates were washed and XTT-phenazine methyl sulfate (PMS) was added to enable counting of the number of live bacteria in biofilms using a microplate reader. XTT assays were also used to explore the effects of NDYP on mature MRSA biofilms: 100 μL of bacterial culture were added to wells of 96-well plates. Bacteria were cultured in the plates for 24 h, and then drug solution was added. The plates were cultured for another 24 h, and then XTT-PMS was added to detect the number of live bacteria in the biofilms. Scanning electron microscopy (SEM) was used to observe the effects of NDYP on mature MRSA biofilms: washed and sterilized glass coverslips were added to 24-well plates. Bacterial culture was added. After 24 h of incubation, drug solution was added. After another 24 h of incubation, the samples were observed by SEM.
Results:
XTT assays showed that the number of live bacteria in both developing and mature MRSA biofilms decreased significantly (P < 0.01) after the administration of NDYP. SEM images showed that NDYP could destroy the structure of the bacteria and resulted in uneven thickness of MRSA biofilms.
Conclusion:
In vitro, NDYP has obvious inhibitory effects on the formation of MRSA biofilms and on mature biofilms.
Insights
New Dayuan powder (NDYP) effectively inhibits Methicillin-resistant Staphylococcus aureus (MRSA) biofilms. This study demonstrates NDYP
Area of Science:
- Microbiology
- Pharmacology
- Biotechnology
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat due to its resistance to antibiotics.
- Bacterial biofilms contribute to persistent infections and treatment challenges.
- Novel therapeutic agents are needed to combat MRSA infections.
Purpose of the Study:
- To evaluate the in vitro efficacy of New Dayuan powder (NDYP) against MRSA biofilms.
- To assess the impact of NDYP on both developing and mature MRSA biofilms.
- To investigate the structural effects of NDYP on MRSA biofilms.
Main Methods:
- 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assays were employed to quantify live bacteria in biofilms.
- Scanning electron microscopy (SEM) was utilized to visualize the structural integrity of MRSA biofilms.
- NDYP was tested on both developing and mature MRSA biofilms under in vitro conditions.
Main Results:
- XTT assays revealed a significant reduction in viable bacteria within both developing and mature MRSA biofilms upon NDYP treatment (P < 0.01).
- SEM analysis indicated that NDYP disrupts bacterial cell structure and leads to irregular biofilm thickness.
- NDYP demonstrated potent antimicrobial activity against MRSA biofilms.
Conclusions:
- New Dayuan powder exhibits significant inhibitory effects on MRSA biofilm formation and existing mature biofilms in vitro.
- NDYP presents a promising therapeutic candidate for managing MRSA infections associated with biofilms.
- Further research into NDYP's mechanism of action and in vivo efficacy is warranted.
